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Improvement in correlation microscopy: Nikon microscope integration with CrestOptics spinning disk and super resolution module.

Zorloni A., Oggioni M., Bacchi F.
  Giovedì 15/09   15:00 - 18:30   Aula E - Rosalind Franklin   V - Biofisica e fisica medica   Presentazione
Correlative microscopy has recently become an important tool in biophysics to obtain live imaging of subcellular dynamics structures, like organelles, vesicles, cytoskeleton filaments, with high spatial and temporal resolution and low photon burden. To answer these requirements, CrestOptics combined a Spinning Disk confocal system (V3) with a Structured Illumination microscopy one (DeepSIM). V3 features micro-lenses--based homogeneous illumination, high temporal resolution (up to $500 {fps}$) with FOV25 and dual cameras setup to get simultaneous multi-color acquisitions ({400\mbox$--$750 ${nm}$) at high confocal throughput and speed, with minimum photobleaching. With such technology, fast dynamic events are consistently tracked in real-time and in large tissue sections. DeepSIM doubles the confocal spatial resolution ({100 ${nm}$ lateral) to resolve fine subcellular details and specifically designed masks of micro lenses and pinholes generate 2D-lattice illumination patterns to scan the sample at depth penetration comparable with confocal, with temporal resolution higher than $10 {fps}$. These aspects altogether foster the seamless integration of the two imaging modalities and their meaningful data correlation.